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KMID : 0356420080260030136
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Journal of Korean Andrology
2008 Volume.26 No. 3 p.136 ~ p.141
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The Effect of Human Umbilical Cord Blood Derived Mesenchymal Stem Cell Therapy in Rat Model of Cavernosal Nerve Injury
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Choi Ah-Ram
Kim Sung-Dae
Sohn Dong-Wan
Kim Doo-Bae
Kim Hyun-Woo
Cho Soo-Yeun
Cho Yong-Hyun
Kim Sae-Woong
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Abstract
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Purpose: To find out the effect of transplanted umbilical cord blood derived human mesenchymal stem cells (hUCB- MSCs) in the rat model of bilateral cavernosal nerve injury (BCNX)
Materials and methods: Male Sprague Dawley rats were divided into three groups: Group I (7 rats, Control, Sham operation) had only bilateral cavernosal nerve crushing injury. PKH-26 fluorescent cell linker was labeled to hUCB- MSCs Group II (7 rats) had transplantation of hUCB- MSCs (3×105cells/50μl PBS) into MPG after BCNX. Group III (7 rats) had transplantation of hUCB- MSCs (3×105cells/50μl PBS) into the CC after BCNX. We detected the PKH-26 labeled MSCs in Group II, and III at four weeks after transplantation. Peak intracavernosal pressure (ICP) and ICP/mean arterial pressure (MAP) were recorded after pelvic nerve stimulation at 12 weeks after transplantation.
Results: PKH-26 labeled hUCB-MSCs were detected in MPG and CC of group II and III at four weeks after transplantation. The peak ICP was significantly higher in the Group II (69.6±5.9mmHg) and Group III (59.7±7.6mmHg) compared to Group I40.6±10.5mmHg) at 12 weeks after transplantation. The rates of ICP/MAP were significantly higher in Group II (32.2±6.4%) and Group III (57.8±4.9%) compared to Group I (49.5±2.9%) at 12 weeks after transplantation.
Conclusions: The intracavernosal and major pelvic ganglion transplantation of hUCB- MSCs improve erectile function in a rat model of neurogenic impotence.
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KEYWORD
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Erection, Corpus cavernosum, Human mesenchymal stem cells
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